Intestinal Content Microbiota Analysis

Intestinal Content Microbiota Analysis

An internationally recognized testing institution, assisting enterprises in achieving technological advancement.

Reasons for choosing our testing services

ZHONGXI Testing has obtained inspection qualification certifications from multiple countries and regions worldwide. We possess a senior testing team and advanced testing methods, providing independent, impartial, and professional third-party verification services for global carbon projects.

Internationally recognized authority

Internationally recognized authority

Certified by multiple international standards such as CNAS, VCS, and GS, with reports universally applicable worldwide.

Global service capability

Global service capability

Covering 140+ countries and regions, it supports on-site detection and remote verification in multiple languages.

Professional experimental methods

Professional experimental methods

Adopt standard experimental methods to ensure accurate and reliable data.

Evaluating third-party partners for microbiota analysis requires a clear understanding of the testing scope and capabilities. Our laboratory offers comprehensive intestinal content microbiota analysis services, accredited by CNAS and CMA, to meet the needs of researchers, engineers, and quality managers. These services are designed to provide detailed insights into microbial composition and diversity, enabling informed decision-making in various applications.

Testing Scope

Our intestinal content microbiota analysis services are applicable to a wide range of products and materials, including but not limited to:

  • Animal intestinal content samples (mammals, birds, fish)
  • Human clinical samples for research purposes
  • Food products containing microbial cultures
  • Biotechnology and pharmaceutical samples

These samples are analyzed to assess microbial diversity, abundance, and functional potential. Application scenarios include nutritional research, disease modeling, probiotic evaluation, and environmental microbiology. Commonly referenced standards for these analyses include ISO 16777 for microbial enumeration, ASTM E1857 for sample handling, and EN 16820 for microbial identification techniques.

Testing Items and Parameters

Our intestinal content microbiota analysis includes multiple test items and parameters to provide a comprehensive assessment. Typical test items and their parameters are outlined below:

Test Item Typical Parameters Graded Limit Requirements
Microbial Enumeration CFU/g (colony-forming units per gram), relative abundance (%) Accuracy ±15%, detection limit ≤10³ CFU/g
Microbial Identification 16S rRNA sequencing, qPCR analysis Identification accuracy ≥95%, quantification precision ±10%
Phylogenetic Diversity Alpha diversity (Shannon index), beta diversity (PCA) Reliability coefficient ≥0.85, statistical significance p<0.05
Functional Potential Assessment Metagenomic analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways Functional prediction accuracy ≥80%, pathway coverage ≥90%

Additional parameters can be customized based on specific client requirements. Graded limit requirements ensure consistent quality and compliance with industry standards.

Data and Variation Analysis

In assessing parallel-sample and batch-to-batch variation, our laboratory employs rigorous statistical methods to ensure data reliability. Parallel-sample variation is evaluated using relative deviation, where results from duplicate or triplicate samples are compared. Typically, relative deviation remains within ±10% for microbial enumeration and ±15% for identification processes.

Batch-to-batch variation is analyzed through control samples and reference materials, with acceptance windows defined based on historical performance data. For example, microbial enumeration results are considered acceptable if they fall within a relative deviation of ±20% between batches. Statistical tools such as ANOVA and Tukey’s HSD test are used to identify significant variations. All variation data is reported with confidence intervals and p-values to indicate statistical significance, ensuring clients have a clear understanding of their sample’s reproducibility and consistency.

Send us your testing item list and sample details, and our engineers will confirm the applicable items and turnaround within one working day.

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About Us

Beijing ZKGX Institute of Science and Technology , combining applied research with technological transformation. It has evolved into a comprehensive research institute characterized primarily by a "task-driven disciplines" approach. Approved by relevant authorities, it currently operates as a third-party analytical testing technical service provider. Its affiliated laboratory facilities hold certifications including CMA and CNAS, possess an Experimental Animal Use License, and have achieved triple certification for ISO9001 Quality Management System, ISO14001 Environmental Management System, and ISO45001 Occupational Health and Safety Management System.