Reasons for choosing our testing services
ZHONGXI Testing has obtained inspection qualification certifications from multiple countries and regions worldwide. We possess a senior testing team and advanced testing methods, providing independent, impartial, and professional third-party verification services for global carbon projects.
Internationally recognized authority
Certified by multiple international standards such as CNAS, VCS, and GS, with reports universally applicable worldwide.
Global service capability
Covering 140+ countries and regions, it supports on-site detection and remote verification in multiple languages.
Professional experimental methods
Adopt standard experimental methods to ensure accurate and reliable data.
Professional Yeast Transcriptome Analysis Service – Uncover the Full Regulatory Landscape
We understand that you are searching for yeast transcriptome analysis because you need to move beyond simple viability or activity tests. Whether you are engineering Saccharomyces cerevisiae for higher ethanol tolerance, investigating stress responses in wine fermentation, or characterising non‑conventional yeasts for bioproduction, our high‑depth transcriptome sequencing and custom bioinformatics service reveals every active gene and regulatory network with unprecedented resolution.
What We Deliver – From Raw Reads to Biological Insight
Our standard yeast transcriptome package includes total RNA extraction (including low‑abundance and small RNAs), ribosomal RNA depletion or poly(A) enrichment, strand‑specific library preparation, and 150 bp paired‑end sequencing on NovaSeq X Plus to achieve >80 million reads per sample. For demanding applications, we offer ultra‑deep sequencing (200+ million reads) to detect rare transcripts and isoform switches. We process samples from any growth condition: chemostat, batch culture, biofilm, solid‑state fermentation, or even single yeast cells isolated from complex matrices (grape must, dough, beer, or host tissues).
Advanced Capabilities That Go Far Beyond Standard DE Analysis
You will not just receive a list of up‑ and down‑regulated genes. Our integrated pipeline includes: differential expression analysis (DESeq2/edgeR) with FDR correction, time‑course clustering using maSigPro, enrichment of GO terms and KEGG pathways, transcription factor binding motif discovery (MEME‑ChIP), and co‑expression network construction (WGCNA). For engineering strains, we perform allele‑specific expression analysis to detect copy number variations or cis‑regulatory mutations. We also offer long‑read isoform sequencing (Iso‑Seq on PacBio) to resolve alternative splicing events, a feature rarely studied in yeasts but crucial for genes like HAC1 or stress‑regulated kinases.
What Makes Our Yeast Transcriptome Service Stand Out
1. Yeast‑optimised protocols: We have developed mechanical + enzymatic lysis that works for tough cell walls (e.g., Yarrowia lipolytica, Kluyveromyces marxianus) and for subcellular fractionation (cytoplasmic vs. nuclear RNA). 2. Low input and degraded samples: Our SMART‑based technology works with as little as 100 pg of total RNA and produces high‑quality libraries even from partially degraded material (e.g., old fermenter samples or freeze‑thawed cells). 3. In‑house reference genomes: For non‑model yeasts, we assemble a draft genome from the same RNA‑seq data (or from a small amount of DNA) and then perform transcriptome annotation. 4. Real‑time spike‑ins: We include ERCC spike‑in controls to normalise across different RNA extraction and sequencing batches, ensuring absolute quantification of transcript copy numbers upon request.
Beyond Expression: Functional and Mechanistic Profiling
We understand that many customers need to link transcription to phenotype. Therefore we add optional modules: ribosome profiling (Ribo‑seq) to measure translation efficiency, nascent RNA sequencing (GRO‑seq or TT‑seq) to capture real‑time transcription dynamics, and ATAC‑seq for yeast to map chromatin accessibility changes. All these can be performed on the same biological replicates as your transcriptome. We also provide integrated metabolic modelling (iMM904 or custom) that maps transcriptomic changes onto flux predictions – showing you which pathways are not just transcribed but likely active.
Our Distinct Advantages in Service Delivery
Speed: From yeast pellet to final report in 10‑12 working days for standard RNA‑seq (including bioinformatics). Reproducibility: Each sample is processed with internal replicates, and we supply raw FASTQ, aligned BAM, count matrix, and a fully documented analysis notebook (R Markdown or Python). Expert consultation: Our team includes yeast molecular biologists who have published transcriptome studies on stress memory, flocculation, and heterologous pathway expression. We help you design the experiment (time points, replicates, inhibitors) to avoid common pitfalls. Secure and scalable: We comply with GDPR and data protection, and we can handle projects from 2 to 500+ samples with automated LIMS tracking.
Ready to Decode Your Yeast’s Transcriptome?
Do not settle for partial expression data that ignores low‑copy regulatory RNAs or fails to reveal network logic. Contact our team today to discuss your yeast strain, growth conditions, and biological questions. We offer a free pilot analysis on up to three samples to demonstrate our depth and clarity. Whether you are in brewing, biofuel, synthetic biology, or basic research, our yeast transcriptome analysis will provide the actionable insights you need.