Reasons for choosing our testing services
ZHONGXI Testing has obtained inspection qualification certifications from multiple countries and regions worldwide. We possess a senior testing team and advanced testing methods, providing independent, impartial, and professional third-party verification services for global carbon projects.
Internationally recognized authority
Certified by multiple international standards such as CNAS, VCS, and GS, with reports universally applicable worldwide.
Global service capability
Covering 140+ countries and regions, it supports on-site detection and remote verification in multiple languages.
Professional experimental methods
Adopt standard experimental methods to ensure accurate and reliable data.
Comprehensive Multiplex PCR Detection Service – Simultaneous Targeting of Multiple Genetic Loci in a Single Reaction
You are searching for multiplex polymerase chain reaction (PCR) detection because you need to perform this assay—to screen multiple pathogens, identify genetic variants, or validate gene co‑expression from limited sample material. We provide exactly that service, combining high‑throughput primer engineering with real‑time and end‑point multiplex platforms that deliver reliable, publication‑ready data.
What We Can Detect – Panel Size, Complexity, and Sensitivity
Our multiplex PCR service goes far beyond simple triplex reactions. Using optimized primer design algorithms (Tm normalisation, hairpin avoidance, cross‑dimers screening) and proprietary hot‑start polymerase blends, we routinely run 15‑ to 30‑plex assays in a single tube. We detect viral (SARS‑CoV‑2, influenza, RSV, CMV), bacterial (MRSA, VRE, Borrelia, Legionella), fungal (Candida, Aspergillus), and parasitic targets simultaneously. For human genetics, we offer multiplex SNP genotyping (up to 40 SNPs/well) and CNV detection via multiplex amplicon sequencing. Even transgenic elements (CaMV 35S, NOS, Cry1Ab) in GMO screening are resolved with 0.1% sensitivity.
How Deep We Go – From Rapid Screening to Quantitative Multiplexing
We don’t just report presence/absence. Our service integrates capillary electrophoresis (fragment analysis) with 1‑bp resolution, high‑resolution melting (HRM) for allele discrimination, and digital multiplex PCR (dPCR) that provides absolute copy numbers for up to 5 targets per partition. For complex panels, we apply dual‑label TaqMan® chemistry with wavelength‑separated fluorophores (FAM, HEX, ROX, Cy5, and ATTO™ dyes), achieving linear dynamic ranges from 10² to 10⁶ copies/μL. Our mass spectrometry‑based multiplex (MassARRAY®) pushes to 50‑plex with single‑base extension precision.
Why Our Multiplex PCR Service Leads – Unmatched Specificity & Workflow Integration
1. Rigorous primer validation: Every new panel goes through in silico specificity screening (NCBI BLAST, PrimeTime) followed by wet‑lab cross‑reactivity testing against 200+ non‑target genomes.
2. Inhibition‑resistant chemistry: Our master mix tolerates up to 20% carryover of blood, soil, or plant lysate, reducing failed reactions and re‑extractions.
3. Full automation & traceability: We use liquid‑handling robots and barcoded 384‑well plates to run 1,000+ samples per day with LIMS‑recorded chain of custody.
4. Flexible reporting: You receive Ct values, melt peaks, fragment electropherograms, or digital copy numbers – all with internal controls (IC) and no‑template controls (NTC) to exclude false positives.
5. Rapid turnaround & support: Routine 5‑plex detection: 24‑48 hours; custom high‑plex panels (20+ targets): 5‑7 business days. Our bioinformaticians also help design your next panel free of charge with a pilot test.
Who Depends on Our Multiplex PCR – Real‑World Applications
Public health labs use our respiratory virus panel (18 targets) to cut testing costs by 70%. Agricultural biotech companies verify seed purity with our 12‑plex GMO event assay. Clinical researchers detect antibiotic resistance genes (blaCTX‑M, mecA, vanA/B) directly from positive blood cultures in 3 hours. One industrial client discovered a low‑abundance co‑infection (Bordetella holmesii masked by B. pertussis) only because our 20‑plex panel included both – a finding missed by commercial singleplex kits.
Ready to Run Your Multiplex PCR Detection?
Send us DNA/cDNA (≥1 ng/μL, any volume), swabs, tissues, or even crude lysates. We perform extraction, multiplex setup, amplification, analysis, and a full interpretive report – all in one laboratory. Request a free consultation for your target list; we will confirm the optimal multiplex strategy within 24 hours.