Reasons for choosing our testing services
ZHONGXI Testing has obtained inspection qualification certifications from multiple countries and regions worldwide. We possess a senior testing team and advanced testing methods, providing independent, impartial, and professional third-party verification services for global carbon projects.
Internationally recognized authority
Certified by multiple international standards such as CNAS, VCS, and GS, with reports universally applicable worldwide.
Global service capability
Covering 140+ countries and regions, it supports on-site detection and remote verification in multiple languages.
Professional experimental methods
Adopt standard experimental methods to ensure accurate and reliable data.
Targeted Brucella Detection – High‑Sensitivity Molecular & Serological Identification Service
You are searching for targeted Brucella detection because you need to perform this assay—whether to confirm infection in livestock or humans, screen bulk milk or meat products, monitor environmental samples from endemic regions, or differentiate vaccine strains from field isolates. We provide a complete detection service that delivers unambiguous genus‑ and species‑level identification, quantitation, and strain characterisation using validated molecular, immunological, and culture‑based platforms.
What We Detect – From Genus‑Specific Targets to Species & Biovar Discrimination
Our targeted Brucella detection goes far beyond conventional Rose Bengal or agglutination tests. We deploy real‑time PCR (qPCR) targeting the BCSP31, IS711, and per genes with analytical sensitivity of 1‑5 genome equivalents per reaction. For species identification, we run multiplex qPCR discriminating Brucella abortus, B. melitensis, B. suis, B. canis, B. ovis, and B. neotomae in a single well. We also perform AMOS‑PCR (for abortus, melitensis, ovis, suis) and MLVA (multiple‑locus variable‑number tandem repeat analysis) with 16 markers to achieve strain‑level resolution for epidemiological tracing. For vaccine differentiation, our SNP‑based assays distinguish S19, RB51, Rev.1 and rough vaccine strains from wild‑type Brucella – critical for post‑vaccination surveillance.
How Deep We Go – From Direct Sample Testing to Isolation & Full Genotyping
We don't just report “positive/negative”. Our service integrates direct PCR from clinical, food, or environmental samples without culture (blood, serum, milk, vaginal swabs, aborted foetal tissues, meat juice, soil swabs) with internal inhibition control and host DNA normalisation. For culture‑confirmed isolates, we provide whole‑genome sequencing (WGS, 100× coverage) with core genome MLST (cgMLST) to map phylogenetic placement, antimicrobial resistance genes, and virulence factor profiles. Our digital droplet PCR (ddPCR) delivers absolute quantification of Brucella DNA in low‑bioburden samples (e.g., environmental swabs) down to 0.5 copy/μL. For serological confirmation, we offer iELISA, fluorescence polarisation assay (FPA), and complement fixation test (CFT) with standardised Brucella lipopolysaccharide (LPS) and smooth/rough antigen panels – all ISO 17025 validated.
Why Our Targeted Brucella Detection Stands Out – Ultra‑Specificity, Biosafety Compliance & Rapid Turnaround
1. Unmatched specificity: All our primers/probes are in silico and experimentally validated against 50+ Brucella strains and 200+ closely related bacteria (Ochrobactrum, Yersinia enterocolitica O:9, Francisella). Cross‑reaction is zero for non‑Brucella – false positives eliminated.
2. Biosafety level 3 (BSL‑3) capable: For culture and live agent work, our facility is fully BSL‑3 certified. We handle inactivated samples for molecular testing under BSL‑2+ enhanced protocols with HEPA filtration and negative pressure.
3. Multi‑matrix validated protocols: We have validated extraction and amplification for whole blood (EDTA/sodium citrate), serum, fresh/frozen tissues, formalin‑fixed paraffin‑embedded (FFPE) blocks, milk powder, cheese, vaginal swabs (in Amies or Cary‑Blair medium), and environmental dust wipes.
4. Rapid answers: Direct qPCR from samples: 4‑6 hours; species ID plus vaccine differentiation: 24 hours; MLVA genotyping: 48 hours; WGS from isolate: 5‑7 business days.
5. Regulatory & export support: Our reports meet OIE (World Organisation for Animal Health) standards for international trade of livestock and animal products. We also support public health investigations with CDC‑compatible reporting templates.
Who Trusts Our Targeted Brucella Detection – Real‑World Impact
Veterinary authorities in brucellosis‑endemic regions use our service to screen 10,000+ bulk milk samples per month – we identified sub‑clinical shedding in 3% of vaccinated herds missed by serology alone. A food safety laboratory sent us artificially contaminated soft cheese; we detected as few as 10 CFU/g of B. melitensis within 6 hours. An international disease surveillance program relied on our MLVA‑16 genotyping to link human cases to a single goat farm source – enabling targeted culling and control. We also helped a reference lab differentiate B. abortus S19 vaccine strain from a field outbreak strain when routine PCR gave ambiguous results.
Ready to Run Your Targeted Brucella Detection?
Send us whole blood (≥1 mL EDTA), serum (≥500 µL), milk (≥2 mL), tissue (≥1 g), swabs (in transport medium), or environmental samples (soil/dust, ≥5 g). We will perform sample inactivation, DNA/RNA extraction, targeted qPCR (genus/species/vaccine), optional MLVA or WGS genotyping, and a full interpretative report – all in as little as 6 hours for urgent cases. Request a free consultation; we will advise on sample collection, shipping (UN3373 Category B), and the optimal detection panel for your surveillance or diagnostic need.